Coelenterazine f

Name: Coelenterazine f

CAS Registry Number: 123437-16-1

CA Index Name: Imidazo[1,2-α]pyrazin-3(7H)-one, 2-[(4-fluorophenyl)methyl]-6-(4-hydroxyphenyl)-8-(phenylmethyl)-

Other Names: CLZN-f; Coelenterazine f

Merck Index Number: Not listed

Chemical / Dye Class: Heterocycle; Imidazo[1,2-α]pyrazin-3(7H)-one

Molecular Formula: C₂₆H₂₀FN₃O₂

Molecular Weight: 425.45

Physical Form: Orange powder

Solubility: Soluble in ethanol, methanol

Melting Point: > 200 °C

Boiling Point: 596.7 ± 60.0 °C, pressure: 760 Torr

pK_a: 10.48 ± 0.30, most acidic, temperature: 25 °C; 6.49 ± 0.60, most basic, temperature: 25 °C

Absorption (λ_max): 437 nm

Emission (λ_max): 472 nm

Synthesis: Synthetic methods

Staining Applications: Calcium ions

Biological Applications: Calcium indicator; assaying luminescent enzyme; measuring luciferase activity; as a substrate for luciferase; screening HIV-1 protease inhibitors

Industrial Applications: Not reported

Safety / Toxicity: No data available

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