Coelenterazine h
Name: Coelenterazine h
CAS Registry Number: 50909-86-9
CA Index Name: Imidazo[1,2-α]pyrazin-3(7H)-one, 6-(4-hydroxyphenyl)-2,8-bis(phenylmethyl)-
Other Names: CLZN-h; 2-Deoxycoelenterazine; Coelenterazine h; Luciferin; Luciferin (Renilla); Renilla luciferin; h-Coelenterazine
Merck Index Number: Not listed
Chemical / Dye Class: Heterocycle; Imidazo[1,2-α]pyrazin-3(7H)-one
Molecular Formula: C26H21N3O2
Molecular Weight: 407.46
Physical Form: Yellow powder
Solubility: Soluble in methanol, ethanol
Melting Point: > 200 °C
Boiling Point: 593.5 ± 60.0 °C, pressure: 760 Torr
pKa: 10.48 ± 0.30, most acidic, temperature: 25 °C; 6.15 ± 0.60, most basic, temperature: 25 °C
Absorption (λmax): 437 nm
Emission (λmax): 466 nm
Synthesis: Synthetic methods
Staining Applications: Calcium ions
Biological Applications: Calcium indicator; assaying luminescent enzyme; measuring luciferase activity; as a substrate for luciferase; screening HIV-1 protease inhibitors; bioluminescence resonance energy transfer (BRET) detection system
Industrial Applications: Not reported
Safety / Toxicity: No data available
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