Ethidium Bromide
Name: Ethidium Bromide
CAS Registry Number: 1239-45-8
CA Index Name: Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1)
Other Names: 3,8-Diamino-5-ethyl-6-phenylphenan-thridinium bromide; Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide; 2,7-Diamino-10-ethyl-9-phe-nylphenanthridinium bromide; 2,7-Diamino-9-phenyl-10-ethylphenanthridinium bromide; 2,7-Diamino-9-phe-nylphenanthridine ethobromide; Dromilac; Ethidium bromide; Homidium bromide
Merck Index Number: 4731
Chemical / Dye Class: Phenanthridine
Molecular Formula: C21H20BrN3
Molecular Weight: 394.31
Physical Form: Dark red crystals
Solubility: Soluble in water, chloroform, dimethyl sulfoxide, ethanol
Melting Point: 238-240 °C
Absorption (λmax): 518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm
Emission (λmax): 605 nm, 620 nm
Synthesis: Synthetic methods
Staining Applications: Nucleic acids; cells; HeLa cells; bacteria
Biological Applications: Nucleic acid hybridization; detecting nucleic acids, cells, cancer cells, human cytomegalovirus, hydrogenase A (hydA) of Clostridia, influenza A virus, oligonucleotides, viable Plesiomonas shigelloides; apoptosis assay; nucleic acid amplification; nucleic acid quantification; treating diseases associated with androgens, cancer, plasma cell disorders, neurodegenerative diseases
Industrial Applications: Not reported
Safety / Toxicity: Carcinogenicity; cytotoxicity; DNA damage; embryotoxicity; genotoxicity; mutagenicity; neurotoxicity; phototoxicity
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