Dihydrorhodamine 123

Name: Dihydrorhodamine 123

CAS Registry Number: 109244-58-8

CA Index Name: Benzoic acid, 2-(3,6-diamino-9H-xanthen-9-yl)-, methyl ester

Other Names: D 23806; D 632; Dihydrorhodamine 123

Merck Index Number: Not listed

Chemical / Dye Class: Xanthene

Molecular Formula: C₂₁H₁₈N₂O₃

Molecular Weight: 346.38

Physical Form: Light red or pinkish-white powder

Solubility: Soluble in N,N-dimethylformamide, dimethyl sulfoxide, methanol

Melting Point: 163-165 °C

Boiling Point: 526.9 ± 50.0 °C, pressure: 760 Torr

pK_a: 4.70 ± 0.40, most basic, temperature: 25 °C

Absorption (λ_max): 289 nm

Synthesis: Synthetic methods

Staining Applications: Mitochondria; blood samples; leukocytes; cells; microbes; granulocytes; epithelial cells; myeloid cells; neutrophils

Biological Applications: Generating, detecting & measuring reactive oxygen species; generating, detecting & measuring reactive nitrogen species; detecting phosphates, peroxynitrile; measuring respiratory burst; chronic granulomatous disease DHR assay; screening antioxidant activity assay; peroxidase substrate

Industrial Applications: Not reported

Safety / Toxicity: Cytotoxicity; hepatotoxicity; nephrotoxicity; neurotoxicity; skin toxicity

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