Dihydrorhodamine 123
Name: Dihydrorhodamine 123
CAS Registry Number: 109244-58-8
CA Index Name: Benzoic acid, 2-(3,6-diamino-9H-xanthen-9-yl)-, methyl ester
Other Names: D 23806; D 632; Dihydrorhodamine 123
Merck Index Number: Not listed
Chemical / Dye Class: Xanthene
Molecular Formula: C21H18N2O3
Molecular Weight: 346.38
Physical Form: Light red or pinkish-white powder
Solubility: Soluble in N,N-dimethylformamide, dimethyl sulfoxide, methanol
Melting Point: 163-165 °C
Boiling Point: 526.9 ± 50.0 °C, pressure: 760 Torr
pKa: 4.70 ± 0.40, most basic, temperature: 25 °C
Absorption (λmax): 289 nm
Synthesis: Synthetic methods
Staining Applications: Mitochondria; blood samples; leukocytes; cells; microbes; granulocytes; epithelial cells; myeloid cells; neutrophils
Biological Applications: Generating, detecting & measuring reactive oxygen species; generating, detecting & measuring reactive nitrogen species; detecting phosphates, peroxynitrile; measuring respiratory burst; chronic granulomatous disease DHR assay; screening antioxidant activity assay; peroxidase substrate
Industrial Applications: Not reported
Safety / Toxicity: Cytotoxicity; hepatotoxicity; nephrotoxicity; neurotoxicity; skin toxicity
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