Calcein
Name: Calcein
CAS Registry Number: 1461-15-0
CA Index Name: Glycine, N,N' -[(3,6 -dihydroxy-3-ox-ospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl) bis(methylene)]bis[N-(carboxymethyl)-
Other Names: Bis [N, N- bis (carboxymethyl) aminome-thyl] fluorescein; Fluorescein, 2 ,7 -bis[[bis(carboxymethyl) amino]methyl]-; Fluorescein-bis(methyliminodiacetic acid); Spiro[isobenzofuran-1(3H),9'-[9H]xanthene], glycine derivative; 2,7-Bis[N,N-bis(carboxymethyl)aminomethy-lene]fluorescein; Acetic acid, [(3,6 -dihydroxy-2 ,7 -fluor-andiyl)bis(methylenenitrilo)]tetra-; Calcein; Fluorescein complexon; Fluorexon; NSC 298193; Oftasceine
Merck Index Number: Not listed
Chemical / Dye Class: Xanthene
Molecular Formula: C30H26N2O13
Molecular Weight: 622.53
Physical Form: Yellow-orange to brown powder
Solubility: Soluble in water, ethanol, N,N-dimethyl formamide, dimethyl sulfoxide
Melting Point: 200 °C
Boiling Point: 952.7 ± 65.0 °C, pressure: 760 Torr
pH Range: 6.0-7.2
Color Change at pH: Weak green fluorescence (6.0) to strong green fluorescence (7.2)
pKa: 6.67
Absorption (λmax): 494 nm
Emission (λmax): 517 nm
Synthesis: Synthetic methods
Staining Applications: Calcium ions; fluoride ions; iron ions; mercury ions; peptides; proteins; antibodies; atherosclerotic plaque; bone; cells; fish; liposomes; neurons; skin; tumor cells; inflammations; lymphokines; hepatocytes
Biological Applications: Calcium indicator; fluoride indicator; iron indicator; mercury indicator; detecting nucleic acids, proteins; treating osteoporosis; drug delivery systems
Industrial Applications: Chemical mechanical polishing; coatings; photoresists
Safety / Toxicity: Acute toxicity; cytotoxicity; mutagenicity; neurotoxicity; ophthalmotoxicity
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