Calcein AM
Name: Calcein AM
CAS Registry Number: 148504-34-1
CA Index Name: Glycine, N,N'-[[30,60-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, 1,1'-bis[(acetyloxy)methyl] ester
Other Names: Calcein O, O'-diacetate tetrakis(acetoxy-methyl)ester; Glycine, N,N'-[[3',6'-bis(acetyloxy)-3-ox-ospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl] bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoe-thyl]-, bis[(acetyloxy)methyl] ester; Spiro[isoben-zofuran-1(3H),9'-[9H]xanthene], glycine derivative; Calcein AM; Calcein acetoxymethyl ester; NSC 689290
Merck Index Number: Not listed
Chemical / Dye Class: Xanthene
Molecular Formula: C46H46N2O23
Molecular Weight: 994.86
Physical Form: Colorless powder
Solubility: Soluble in dimethyl sulfoxide
Melting Point: > 250 °C
Boiling Point: 982.7 ± 65.0 °C, pressure: 760 Torr
pKa: 2.66 ± 0.50, most basic, temperature: 25 °C
Absorption (λmax): < 300 nm
Synthesis: Synthetic methods
Staining Applications: Calcium ions; zinc ions; bacteria; fungi; yeast; cells; cornea; erythrocytes; lymphocytes; microorganisms; neurons; Schwann cells; sperms; tissues
Biological Applications: Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes; multi-drug resistance probes; treating atherosclerosis, cancer; ischemic disease
Industrial Applications: Not reported
Safety / Toxicity: Carcinogenicity; cytotoxicity; fish toxicity; nanoparticle toxicity; neurotoxicity; phototoxicity; ophthalmotoxicity; reproductive toxicity
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