Blue Tetrazolium (BT)
Name: Blue Tetrazolium (BT)
CAS Registry Number: 1871-22-3
CA Index Name: 2H-Tetraolium, 2,2-(3,3-dimethoxy [1,1'-biphenyl]-4,4'-diyl)bis[3,5-diphenyl-, chloride (1:2)
Other Names: 2H-Tetrazolium, 2,2-(3,3 -dimethoxy [1,1'-biphenyl]-4,4'-diyl)bis[3,5-diphenyl-, dichloride; 2H-Tetrazolium,3,3'-(3,3'-dimethoxy-4,4'-biphenylylene) bis[2,5-diphenyl-, dichloride; 3,3 -(3,3 -Dimethoxy-4,4 -biphenylylene)bis[2,5-diphenyl-2H-tetrazolium chloride]; 2,2',5,5'-Tetraphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 2,2'-(m,m'-Dimethoxy-p, p0-biphenylene)bis(3,5-diphenyltetrazolium chloride); 3,3 -(3,3 -Dimethoxy-4,4 -biphenylene)bis[2,5-di-phenyl-2H-tetrazolium chloride]; 3,3 -Dianisolebis[4,4 -(3,5-diphenyl)tetrazolium chloride]; BT; BT (dye); Blue tetrazolium; Blue tetrazolium chloride; Ditetrazolium chloride; NSC 27623; Tetrazolium blue; Tetrazolium blue chloride
Merck Index Number: 9244
Chemical / Dye Class: Tetrazolium salt
Molecular Formula: C40H32Cl2N8O2
Molecular Weight: 727.64
Physical Form: Lemon yellow crystals or powder
Solubility: Soluble in water; freely soluble ethanol, methanol, chloroform; insoluble in acetone, ether, ethyl acetate
Melting Point: 242-245 °C (decompose)
Absorption (λmax): 253 nm
Synthesis: Synthetic methods
Staining Applications: Fungi; motoneurons
Biological Applications: Cellular response evaluation assays; microbial growth assays; tannins assays; anti-cancer agents; diagnostic test strips; detecting lactate dehydrogenase (LDH) isoenzymes, gamma-hydroxybutyric acid (GHB); measuring ATP, number of soil microorganisms, niacin; treating cancer
Industrial Applications: Electrochromic displays; recording materials; toner; photochromic devices; thin-film sensor; photographic materials
Safety / Toxicity: Cytotoxicity; dental toxicity; hepatotoxicity; in vitro toxicity
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