Dihydroethidium
Name: Dihydroethidium
CAS Registry Number: 104821-25-2
CA Index Name: 3,8-Phenanthridinediamine, 5-ethyl-5,6-dihydro-6-phenyl-
Other Names: 3,8-Phenanthridinediamine, 5-ethyl-5,6-dihydro-6-phenyl-, (±)-; Dihydroethidium; Hydroethi-dine; HE; HYD; PD-MY 003
Merck Index Number: Not listed
Chemical / Dye Class: Phenanthridine
Molecular Formula: C21H21N3
Molecular Weight: 315.41
Physical Form: Pink to dark brown powder
Solubility: Soluble in N,N-dimethylformamide, dimethyl sulfoxide, acetonitrile, methanol, chloroform
Melting Point: 580.4 ± 50.0 °C, pressure: 760 Torr
pKa: 5.30 ± 0.40, most basic, temperature: 25 °C
Absorption (λmax): 355 nm
Emission (λmax): 420 nm
Synthesis: Synthetic methods
Staining Applications: Superoxide ions; nucleic acids; cells; myocardium; bacteria; prokaryotes
Biological Applications: Apoptosis assay; generating and detecting reactive oxygen species (ROS); detecting nucleic acids, cells; measuring respiratory burst; superoxide indicator; viability assay
Industrial Applications: Assaying reactive oxidants in smoke
Safety / Toxicity: Metabolic toxicity; neutotoxicity; parasite toxicity; vascular toxicity
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