DAF FM
Name: DAF FM
CAS Registry Number: 254109-20-1
CA Index Name: Spiro[isobenzofuran-1(3H),9'-[9H] xanthen]-3-one, 4-amino-2',7'-difluoro-3',6'-dihydroxy-5-(methylamino)-
Other Names: 4-Amino-5-methylamino-2',7'-difluoro-fluorescein; DAF-FM
Merck Index Number: Not listed
Chemical / Dye Class: Xanthene
Molecular Formula: C21H14F2N2O5
Molecular Weight: 412.34
Physical Form: Solid
Solubility: Soluble in dimethyl sulfoxide, methanol
Melting Point: 265 °C
Boiling Point: 693.7 ± 55.0 °C, pressure: 760 Torr
pKa: 6.88 ± 0.20, most acidic, temperature: 25 °C; 3.99 ± 0.20, most basic, temperature: 25 °C
Absorption (λmax): 487 nm, 495 nm
Emission (λmax): 515 nm
Synthesis: Synthetic method
Staining Applications: Nitric oxide ions
Biological Applications: Nitric oxide indicator; detecting microorganisms; measuring Gα i-coupled or Gα o-coupled receptors activity
Industrial Applications: Not reported
Safety / Toxicity: No data available
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