Tetranitro Blue Tetrazolium (TNBT)
Name: Tetranitro Blue Tetrazolium (TNBT)
CAS Registry Number: 1184-43-6
CA Index Name: 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy [1,1'-biphenyl]-4,4'-diyl)bis[3,5-bis(4-nitrophenyl)-, chloride (1:2)
Other Names: 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy [1,1'-biphenyl]-4,4'-diyl)bis[3,5-bis(4-nitrophenyl)-, dichloride; 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylylene)bis[2,5-bis(p-nitrophenyl)-, dichloride; [3,30-(3,3'-Dimethoxy-4,4'-biphenylylene)bis[2,5-bis(p-nitrophenyl)-2H-tetrazolium chloride]]; 2,2',5,5'-Tetra-p nitrophenyl-3,3'-(3,3'-dimethoxy-4,4'-biphenylene)dite-trazolium chloride; 2,2',5,5'-Tetrakis(p-nitrophenyl)-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 2,2',5,5'-Tetrakis-p-(nitrophenyl)-3,3'-bis(3,3'-di-methoxy-4,4'-diphenylene)ditetrazolium chloride; Blue p-nitrotetrazolium chloride; NSC 121208; Nitroblue monotetrazolium chloride; TNBT; Tetranitro Blue Tetra-zolium; Tetranitro-BT; Tetranitroblue tetrazolium chloride; Tetranitrotetrazolium blue
Merck Index Number: Not listed
Chemical / Dye Class: Tetrazolium salt
Molecular Formula: C40H28Cl2N12O10
Molecular Weight: 907.63
Physical Form: Yellow crystals or powder
Solubility: Soluble in water, ethanol, methanol, N,N-dimethylformamide
Melting Point: 170 °C (decompose)
Absorption (λmax): 279 nm
Synthesis: Synthetic methods
Staining Applications: Cancer tissues; enzymes; β-galactosidase; glucose-6-phosphate dehydrogenase; motor nerve terminals
Biological Applications: Diagnosis of bacterial vaginosis; detecting alkaline phosphatase, gamma-hydroxy-butyric acid (GHB), succinate dehydrogenase activity; generating and detecting reactive oxygen species; treating cancer
Industrial Applications: Recording materials; steel products; toner
Safety / Toxicity: Bacterial toxicity
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