Safranin O

Name: Safranin O

CAS Registry Number: 477-73-6

CA Index Name: Phenazinium, 3,7-diamino-2,8-di-methyl-5-phenyl-, chloride (1: 1)

Other Names: C.I. Basic Red 2; Phenazinium, 3,7-diamino-2,8-dimethyl-5-phenyl-, chloride; Safranine O; 2,8-Dimethylphenosafranine; 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride; Basic Pink; Basic Red 2; Brilliant Safranine BR; Brilliant Safranine G; Brilliant Safranine GR; C.I. 50240; Calcozine Red Y; Cotton Red; Duasyn Basic Red TH; Gossypimine; Hidaco Safranine; Leather Red HT; Lowacryl Red 2; Lowacryl Red 2 Conc; Mitsui Safranine T; Nippon Kagaku Safranine GK; Nippon Kagaku Safranine T; Safranin; Safranin O; Safranin T; Safranine; Safranine A; Safranine B; Safranine G; Safranine GF; Safranine J; Safranine OK; Safranine OK 70:100; Safranine Superfine G; Safranine T; Safranine TH; Safranine TN; Safranine TS; Safranine Y; Safranine YN; Safranine Zh; Tolusafranine

Merck Index Number: Not listed

Chemical / Dye Class: Phenazine

Molecular Formula: C₂₀H₁₉ClN₄

Molecular Weight: 350.84

Physical Form: Dark red to dark green powder

Solubility: Soluble in water, ethanol, ethylene glycol, methyl cellosolve, pyridine

Melting Point: > 240 °C (decompose)

pK_a: 6.4

Absorption (λ_max): 530 nm

Synthesis: Synthetic methods

Staining Applications: Antigen; bacteria; brain; cellulose; lignin; mitochondria; nucleated and non-nucleated blood cells; nucleic acids; proteins; spinal cord; hairs

Biological Applications: Hematotoxicity assays; measuring membrane potential; detecting microorganisms; treating diabetes-associated pain, mechanical allodynia, oncological diseases; food packaging materials

Industrial Applications: Photogalvanic cells; polymeric electro-mechanic devices; recording material; inks; textiles

Safety / Toxicity: Bacterial toxicity; carcinogenicity; environmental toxicity; microbial toxicity; mitochondrial toxicity; mutagenicity; nucleic acid damage

Certification / Approval: Certified by Biological Stain Commission (BSC)

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