Propidium Iodide
Name: Propidium Iodide
CAS Registry Number: 25535-16-4
CA Index Name: Phenanthridinium, 3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenyl-, iodide (1:2)
Other Names: 3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide; Phenanthridinium, 3,8-diamino-5-[3-(diethylmethylammonio) propyl]-6-phenyl-, diiodide; Ammonium, [3-(3,8-diami-no-6-phenyl-5-phenanthridinio)propyl]diethylmethyl-, diiodide; 3,8-Diamino-5-(diethylmethylaminopropyl)-6-phenylphenanthridinium diiodide; PI; Propidium; Pro-pidium diiodide; Propidium iodide
Merck Index Number: Not listed
Chemical / Dye Class: Phenanthridine
Molecular Formula: C27H34I2N4
Molecular Weight: 668.39
Physical Form: Red powder
Solubility: Soluble in water, N,N-dimethylformamide, dimethyl sulfoxide
Melting Point: 220-225 °C (decompose)
Absorption (λmax): 493 nm, 535 nm
Emission (λmax): 636 nm, 617 nm
Synthesis: Synthetic methods
Staining Applications: Nucleic acids; cells; HeLa cells; metastatic cancer cells; stem cells; yeast cells; leukocytes; lymph nodes; lymphoma cell lines; megakaryocyte; microorganisms; parasites; proteins; sperms
Biological Applications: Detecting nucleic acids, cancer cells, spores, activity of hydrolase, microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens, cancer
Industrial Applications: Not reported
Safety / Toxicity: Cytotoxicity; embryotoxicity; mutagenicity; neurotoxicity
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