Methylene Blue Trihydrate
Name: Methylene Blue Trihydrate
CAS Registry Number: 7220-79-3

CA Index Name: Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, hydrate (1:1:3)
Other Names: C.I. Basic Blue 9, trihydrate; C.I. 52015; Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, trihydrate; 3,7-Bis(dimethylamino)phenazathionium chloride trihydrate; Methylene blue trihydrate
Merck Index Number: 6060
Chemical / Dye Class: Phenothiazine
Molecular Formula: C16H18ClN3S3H2O
Molecular Weight: 373.90
Physical Form: Dark green needles or crystals or powder
Solubility: Soluble in water, chloroform; sparingly soluble in ethanol
Melting Point: 190 °C
pKa: 2.6, 11.2
Absorption (λmax): 668 nm, 609 nm
Synthesis: Synthetic methods
Staining Applications: Bacteria
Biological Applications: Diagnosis of tauopathy, Alzheimer’s disease (AD), Pick’s disease; treating prophylaxis; dental materials
Industrial Applications: Thin films; data storage media; photothermographic materials; crayons
Safety / Toxicity: Acute toxicity; cytotoxicity
Certification / Approval: Certified by Biological Stain Commission (BSC)
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